I want to perform pot trails by induction of rust on bean, What is the methodolgy to induce rust? How much time it will taken to appear urediospores on leaves?
By making a fine needle dots/micro pores on the sterilized surface of all leaves. Then spray fresh urediospores mass with the help of microsprayer.cover the entire inoculated leaves with the help of poly bags and make perforation in the poly bags for ventilation. By this way you can also have a pathogenicity.
In the beginning, the samples are collected from infected plants and making of suspended solution of spores rust urediospores using Alatomizr device to spray the solution on the susceptabilty plants, and the plants are placed in rooms with high moisture content and the appropriate degree of temperature
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