Dear all,
I am looking for a protocol to induce insulin resistance in a hypothalamic cell line. Currently I am trying it by treating the cells with palmitic acid over night and challenge them with insulin to look at the phosphorylation of AKT. But the results are not satisfying. Hence I kindly request you to help me in this regard. If there are any other compounds or drugs available to induce insulin resistance kindly let me know. Any help in this regard will be greatly appreciable.
Many thanks in advance.
Cheers
Dear Goutham, I hope that some articles will help you sort out this problem. All the best.
Dear Vladimit many thanks for the information. I know the first one. Thank you again.
I agree with the recommendation to test TNF alpha; also consider IL1 beta and IL6.
Palmitic acid treatment (100 uM) is another possibility.
Overnight treatment with 100 nM insulin may work - has been described to work for cultured cortical neurons; hypothalamic neurons -- possibly.
Good luck.
Thank you tim. I am trying different possible ways as mentioned above. I will update here.
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