Timothy Graham

3 Questions 8 Answers 0 Followers

Questions related from Timothy Graham

Are there theoretical limits to how many viable T cells can be derived from peripheral blood donation (e.g., PBMCs) of a single healthy donor?

Immune oncology drug programs are increasingly moving towards use of allogeneic T cells which can be engineered and used with unmatched recipients. Celyad, for instance, recently got clearance...

08 August 2018 1,622 2 View

Is there a hepatoma cell line that retains many of the functions of primary hepatocytes, but is easier to culture and transfect than HepG2 or Huh7?

We are studying metabolic responses to pyruvate and other glycolysis intermediates. Glucose production capacity is not critical for our studies.

03 March 2015 1,624 6 View

Any suggestion for a reliable protocol for measuring mtDNA content in mouse tissues as a marker of mitochondrial content?

We are working with mice that have impaired autophagy in different tissues. Autophagy is the principal pathway for turnover/degradation of intact mitochondria. We are measuring mitochondrial...

01 January 2014 5,497 19 View