What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Why do my cells die during transfection with JetPei?

Hello everyone, two months ago I did a transfection of HEKs cells to insert a miR using the JetPei reagent. On that occasion, the transfection was highly efficient and the cells were in perfect...

03 February 2021 1,296 6 View

What are the essential elementts of a Nature Neuroscience Paper?

What would land a neuroscience paper in Nature Neuroscience? What is the minimum a paper should have to pass the editorial scrutiny in Nature Neuroscience or the journals around the same cadre?...

01 January 2021 1,510 2 View

Label Propagation - Multi Atlas based 3D Image segmentation implementation. The basics?

How do i get started with the implementation of Label propagation (Multi Atlas based Image segmentation) using sparse coding? The same has been implemented in the paper Representation Learning: A...

17 November 2020 8,569 7 View

Why is the impedance of ECoG electrodes tested at a frequency range from 1 Hz -100 kHz?

I am testing the impedance of a material that will be used for ECoG electrodes. I was told to test it at a frequency range from 1 Hz - 100 kHz but why is this range used? I wasnt able to find a...

08 November 2020 8,089 3 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Paper authorship for students?

I had a few students help me with a simple but time-consuming task. The data they helped with will be used in a scientific paper. The students are part of the Student Research Program (SRP) at my...

11 October 2020 4,514 12 View

How is the quality of archived blood sample for HbA1c test?

HbA1c is an important indicator of long-term glycemic control with the ability to reflect the cumulative glycemic history of the preceding two to three months. Also, HbA1c is measured as the ratio...

16 September 2020 2,423 4 View

How can we explain the decrease in taste in a patient with desminopathy (myofibrillar myopathy)?

A patient with hereditary desminopathy (mutation Thr341Pro DES in a heterozygous state) with disease progression has a significant decrease in taste. How can this fact be explained?

15 September 2020 3,742 4 View

HeLa cells infected with HPV16/18 or not?

I ant to know if HeLa cells line commonly available in cell culture labs is infected with HPV16/18 or not?

01 September 2020 7,286 3 View

What if I can't find k values for n,k files in Atlas, Silvaco ?

Hello, I am looking for simulating GaAs(x)N(1-x) Material is Atlas, But I am not able to find the k values to give in the n,k index files, but I have the data for n. What should I do?

30 July 2020 7,912 2 View

How can i simulate loss in each layer of perovskite in Silvaco TCAD?

I am simulating a Perovskite solar cell to optimize it for best possible efficiency. I want to see losses in each layer of the perovskite solar cell, absorber, HTL and ETL and TCO . Does anybody...

15 June 2020 252 5 View