How can i include GO terms in the process of protein function prediction through domain?
NCDs are increasing alarmingly even in developing countries. Need to take participatory policies for prevent and control of NCDs.
30 July 2024 7,942 3 View
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30 June 2024 4,031 3 View
I am polishing with emery papers with different grit sizes followed by diamond polishing and colloidal sols after ultrasonication with no scratch After that when I go to EBSD CI is not coming....
25 June 2024 2,402 3 View
Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...
23 June 2024 4,763 0 View
23 June 2024 6,583 2 View
In solid ceramic electrolyte what is the Physical significant of area under the curve in dielectric loss tangent studies ??
23 June 2024 4,354 1 View
I am working on polymerizing monomers similar to lactic acid, each containing one carboxylic acid and one alcohol group, through a polycondensation technique. Despite using vacuum conditions...
20 June 2024 2,966 4 View
I am exploring the potential of AI to transform the teaching of linguistics, specifically for English as a Foreign Language (EFL) students. I am interested in understanding how AI can analyze...
10 June 2024 6,836 3 View
How can advanced image processing techniques be used to enhance the quality of medical imaging for early disease detection?
09 June 2024 6,256 0 View
How does the application of generative adversarial networks (GANs) for data augmentation impact the robustness and accuracy of image classification models?
09 June 2024 2,923 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View