Dear Chella,

FT-IR is the best method to distinguish the presence of hydrogen bonding.

The area of 3200-3700 Cm-1 is the frequency to be considered (hydroxyl frequency).

Alcohol

O-H (stretch, H-bonded) 3200-3600 strong, broad

O-H (stretch, free) 3500-3700 strong, sharp

C-O (stretch) 1050-1150 strong

Hoping this will be helpful,

Rafik

I agreed to Mr Rafik, that it is easily identify in thr FT-IR spectra.

If no OH group present in your structure than also it give you broad medium intensity peak in 3500-3350 cm-1 region.

Dear Rafiq,

I agree "O-H (stretch, H-bonded) 3200-3600 strong, broad" and

"O-H (stretch, free) 3500-3700 strong, sharp".

but how to determine the hydrogen bond between two strands of DNA (i.e. between A & T and G & C) and several such hydrogen bonding between several biomolecules such as those seen between few proteins, proteins with polysaccharides, proteins with lipids etc. besides the strong bods seen between O & H in OH or C & H in CH3 or N & H in NH or NH2 etc., through simple analytical methods?