there is no master protocol for endophyte identification, so is there any other protocol for identify endophytic fungi?
Dear Vipin,
The disc incubation method will be able to isolate the endophytic fungi on PDA media. protocol will follow some following basic steps.
Sampling of the interested sample tissue .
Disc preparation.
Surface sterilization bu sodium hypo-chloride or HgCl2.
Disc placement on the suitable media.
Incubation for growth to colony appearance.
For more detail kindly go through the following link.
Good Luck.
http://onlinelibrary.wiley.com/doi/10.1111/j.1574-695X.2006.00155.x/epdf
http://onlinelibrary.wiley.com/doi/10.1046/j.1469-8137.2000.00716.x/epdf
thank you sir for the link
thank you very much sir
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