What is the best procedure of growing tobacco plants in PEG6000 nutrient culture without exposing roots to direct contact with PEG. Does anyone have a protocol for this?
I wish to do similar studies using PEG 6000 as an osmoticum to see seed germination behavior. I have found one study where a fritted glass disk was used to separate the PEG solution from the intended subject (which was a mixture of soil and an absorbent polymer). But there is no specification of its pore size etc. I am still searching. Hope this helps you and please share your suggestions on that counts.