I am working with species of the genus Lathyrus (grasspea), whose seeds are essentially like little (pebble sized) rocks. The goal is to get the seeds to a powder of at least sand-sized coarseness. I have tried the following without success (all with liquid N2):
- mortar & pestle
- bead beater with 3mm steel beads, 30c/s, 10-15min.
Parts of the seed are becoming powderized, but there are still large (2-3mm) chunks that just won't wear down. Are there any suggestions for getting around this problem?
Do you have access to something similar to this:
https://www.ika.com/en/Products-Lab-Eq/Dispersers-Homogenizer-csp-177/ULTRA-TURRAX-Tube-Drive-P-control-Workstation-cpdt-25005836/
If not, you could also try ultrasonic agitation for a prolonged time?
Thank you - we do not have this equipment, but will definitely look into it.
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