03 March 2012 4 836 Report

Immunohistochemistry

More Itopa Ajayi's questions See All
How do you differentiate antidromic from orthodromic signals in the brain?

In studying the physiological interactions between two interconnected brain areas using electrophyiological techniques, how do you differentiate between antidromic and orthodromic signals?

02 March 2014 2,712 5 View

Can anyone help me with information on how to determine neuronal density in an area of the brain?

I wish to determine the neuronal density of a part of the brain using an advanced technique. I am hoping that this coupled with other morphological and electrophysiological investigations may help...

05 June 2013 3,128 10 View

Any suggestions on classical anatomical studies that can be done on the cingulate gyrus?

In recent times, a bit has been done using fMRI to study the functions of the cingulate gyrus. However, this may not be able to completely rule out classical anatomical techniques of histology...

04 May 2013 855 4 View

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Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

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Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

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Can you polarize Jurkat cells into different T helper subsets?

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Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

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Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

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Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

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