Immunohistochemistry
Please specify your question: by any means or only by IHC
Only by ihc
In terms of mouse, B220 or CD19 (for B cells) vs. CD3 (for T cells) staining, then manually counting or using software.
Thanks Gregory. Please do you have the details of any counting software you would recommend?
In studying the physiological interactions between two interconnected brain areas using electrophyiological techniques, how do you differentiate between antidromic and orthodromic signals?
02 March 2014 2,789 5 View
I wish to determine the neuronal density of a part of the brain using an advanced technique. I am hoping that this coupled with other morphological and electrophysiological investigations may help...
05 June 2013 3,233 10 View
In recent times, a bit has been done using fMRI to study the functions of the cingulate gyrus. However, this may not be able to completely rule out classical anatomical techniques of histology...
04 May 2013 962 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...
15 June 2024 3,208 4 View
define cd
06 June 2024 9,533 5 View
I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...
01 June 2024 1,352 0 View
Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...
13 May 2024 2,828 0 View
how can immunological interventions potentially address these issues?
04 April 2024 4,951 1 View
I am a PhD candidate in immunology at AWKUM with a research focus on chronic inflammation, particularly the role of macrophage cells. I am writing to express my keen interest in your research on...
28 March 2024 7,798 0 View
I have several pairs of parameters (obtained from females and males) and want to find the difference in correlation between the two sexes for each parameter, but also want to give a weight so that...
11 March 2024 1,000 4 View