How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

How to generate ECFP hashing folding data?

I'm trying to convert chemical structures to ECFP data. Buy, I have a problem about folding step. I understand all of the process about generating ECFP data though D. Rogers and M. Hahn's paper...

09 January 2021 7,454 2 View

Why train curves are being repeated in this five fold cross validation?

Hello, I am using ...... optimizer = RMSprop(lr=1e-4, decay=1e-6) N_FOLDS = 5 EPOCHS = 20 INIT_LR = 1e-4 Train_Batch_Size = 32 Valid_Batch_Size = 32 decay_rate = 0.95 decay_step =...

30 December 2020 2,519 3 View

How to change material in ANSYS & perform brittle deformation ?

I am currently working on a model for fracture generation due to pressure applied in ANSYS. When I am working with structural steel material it is showing only folding not any fracture initiation...

16 December 2020 3,826 3 View

Why accuracy over each k-fold corssValidation differ alot?

I have a data set with 91 Obervsation with 700 features. I have reduced the dimension of data using PCA? then I split the data set into 70/30. After that applied kfold =10 cross-validations on...

04 December 2020 1,259 4 View

How to get the most out of your qPCR data?

23 October 2020 7,537 3 View

Can I analyse NGS DATA from an EXCEL spreadsheet?

Hi, I have Fold expression of RNAseq data obtained from Ion Proton sequencer and got the fold change data with mean in an excel sheet. now can I analyse this fold change data using any...

20 October 2020 2,240 3 View

Which is the best way to implement 10-fold cross validation for CNN model?

I was performing a binary classification problem with 15000 RGB images using a scratch build CNN model. While it comes to evaluate the model, I can do it in two ways: 1. Splitting data Train...

08 September 2020 8,641 6 View

What is the best force field for proteins with folded and disordered regions?

Hello I want to do MD simulation on a protein model to refine its structure. My protein structure have both folded and intrinsically disordered regions. What force field should i choose for this...

23 August 2020 9,381 5 View

Shall we split the data into 60% of training and 40% of testing. And apply k fold cross validation for training and the best trained model for test?

Is the above method of splitting the data set into taring and testing, and then apply k fold cross validation for training, and then the trained model to classify the test set is right?

04 April 2020 9,040 3 View