i need samples but alignet by pileup formates for hapatocellular carcinoma becouse i dont prefere to work with samtools ,so where can i fined samples of that formate?
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
Hi! I have a question. I was looking into the research question: does mohs micrographic surgery give less recurrences and metastasis than standard excision in the treatment of cuteanous squamous...
17 June 2024 7,769 5 View
Hi everyone! As I previously shared with you, I've started to work with Caco-2 cells and even asked for your help. I recently followed a protocol to extract proteins, but when I collected the...
12 May 2024 6,819 2 View
Dear Colleagues, We plan to isolate and characterise extracellular vesicles released by dying cells during radiotherapy of head and neck squamous cell carcinoma. Given the duration of...
14 April 2024 494 3 View
I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?
28 January 2024 8,818 6 View
I need a MRI images datasets for HCC to extract some features from it , i will be delighted if someone menstion a specific data.
16 January 2024 1,069 1 View
I am looking for TP53 status of various carcinoma cell line from DepMap portal (https://depmap.org/portal/gene/TP53?tab=characterization&characterization=mutation), but could not get the P53...
03 January 2024 1,643 0 View
We are attempting to establish primary cell lines from patient tumor samples of head and neck squamous cell carcinoma (HNSCC). Following transport to LUMS within 30-45 minutes of surgery, the...
01 January 2024 9,097 0 View
Hi, we've been unsuccessful in creating single clones with deletion of a couple of proteins, after testing several sgRNA sequences for each. We are using a lentiviral vector encoding puromycin for...
25 December 2023 2,901 2 View
I really want to know what is the best timing to inject tamoxifen to induce PDAC. My preliminary experiments is not enough to give a suggestion. I have read some papers and they have different...
04 December 2023 9,269 0 View