I am finding a lot of cellular debris in the culture flask? What are the reason behind it?

I am facing a problem that cellular debris observed in my SHSY5Y cells. I have seeded the cells in DMEM media with 10% FBS in T-25/ 60mm dish (antibiotic used PEN-Strepand gentamycin). I have also...

17 January 2021 3,074 3 View

How can we calculate theoretical stacking fault energy of unknown/new complex alloys?

Is there any method/theoretical approach to calculate the stacking fault energy of complex alloys such as high entropy alloys? The theoretical/ approximate value of SFE is needed for alloy design.

16 November 2020 7,074 3 View

Is there any design method for control system without linearization of nonlinear plant?

Usually, a nonlinear plant needs to be linearized before using some design method for control law synthesis. Is there any design method, which does not require a linearization?

14 September 2020 1,268 2 View

LC3 II form remains unchanged but p62 protein is increasing, why?

Hello, I am treating my cells with a compound to study whether the autophagy can be affected or not. Using Western blot, I found that LC3 II form remains unchanged but p62 protein is increasing ....

16 August 2020 8,937 3 View

How do I get the permission for using the images of a published article?

How do I get the permission for using the images of a published article?

03 August 2020 9,083 3 View

How to fill the gap between two contigs?

Hi everyone, We have sequenced genome of data of a comparatively less studied species using Pacbio long read sequencer. Now after initial assembly we compared the contigs with previous version...

25 June 2020 3,442 3 View

How ultrasound and microwave can be effective factors on cotton and wool fabric which help the fabric to improve its dye uptake ability?

How ultrasound and microwave can be effective factors on cotton and wool fabric which help the fabric to improve its dye uptake ability?

15 June 2020 4,541 3 View

How COVID19 would affect the other research areas in Biology?

As the whole world is suffering with COVID19 infection; the research for vaccines are at its peak and so is the funding towards virology projects. Will it have a negative impact on funding in...

25 May 2020 8,579 3 View

How to make ROC plot for the validation of ligand-based-pharmacophore models in discovery studio?

Hello, I already made a ligand-based pharmacophore by utilizing 60 dataset compounds by catalyst module in discovery studio. But I want to explore the ROC curve of that pharmacophore in order to...

02 March 2020 9,044 5 View

How to solve the GROMACS error: The initial cell size (0.657461) is smaller than the cell size limit (0.683200)?

I am simulating a glycerol-water mixture system in a square box of length 3.5 nm. After the NPT step my box size decreased to 2.62984 nm. However, in the beginning of the production run step I...

27 February 2020 2,830 1 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View