db db mice are suitable as a model of insulin resistance and type 2 DM
Are they available in Egypt?
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
I have created a box with a lattice structure but when I make a simulation, I can't select faces for boundary condition. Furthermore, when I export part to ANSYS ,the faces are not defined.
23 May 2024 7,978 0 View
I am using a new system of molecular beam epitaxy (COMPACT 21). the pressure of the growth chamber was 10^-10 torr. suddenly, we found it a round 10^-7 torr. This increase in the pressure happened...
12 February 2024 3,288 0 View
As my article is in public domain can I publish same article in good impact journal
08 February 2024 2,039 1 View
How do limitations in multi-element analysis impact the comprehensive understanding of heavy metal interactions in a given environment?
18 January 2024 3,272 3 View
What is the probability of organic nitrogen transformation to ammonia in these processes partial nitrification, Anammox and aerated filter ?
20 September 2023 2,616 0 View
Some of the most crucial decisions in identifying research gaps involve search strategy decisions taken in doing a systematic review of the literature(SLR). Such decisions influence...
12 September 2023 7,510 1 View
Hi, I want to induce DNA damage using the UV light in the laminar flow hood? I inquire is that possible and what is the required duration? Thanks,
12 September 2023 3,061 1 View
Comparison between Sofosbuvir free regimen and Sofosbuvir based regimens in the treatment of chronic HCV patients with chronic renal failure
03 September 2023 3,474 0 View
Due to false positives, AI writing detectors such as GPTZero, ZeroGPT, and OpenAI's Text Classifier cannot be trusted to detect text composed by ChatGPT. What is your opinion with regard to this...
29 August 2023 3,301 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View