How can I get contig fasta file from ABI chromatogram file? Could anyone help me in this situation?

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Dominique Liger

Hi there,

If you open the abi file with Chromas or FinchTV (free softwares available online) then you can export and save sequences as fasta.

Ram Hari Dahal

Thank you Domanique Liger. I got the sequences as fasta but how could I get a single (contig) fasta from two chromatograms ( Forward and reverse). Anticipating your answer.

M. Luna-Rodríguez

Hello. You can try using the "Mesquite" software.

Thanks Luna. It works