Sirius Red Staining heart. I don't have a clear yellow color. I always get grayish yellow in many areas in one field and this affect analysis with image J is there is any suggestion ?
Regiane R. Santos
Are you using polar birefrigence? if so, you need to adjust the microscope only. If you check only with light microscopy, you will not get this yellow colour out.
0 votes
0 thanks
Mohamed M.S. Gaballa
I'm using light microscope but in the same field l have yellow area and grayish areas so when I use image j to calculate the fibrosis percent I have wrong results
0 votes
0 thanks
Regiane R. Santos
To calculate fibrosis percent, you better use a filter of birefrigence. With this you can even calculate the area from different collagen types and thickness.
0 votes
0 thanks
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining
More Mohamed M.S. Gaballa's questions See All
Similar questions and discussions