06 April 2023 1 1K Report

How can i generate the standard curve of GSH content?

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How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

How to assure whether a regression tree overfit or not by seeing bias-variance value of the model?

Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...

22 February 2021 7,755 2 View

Similar questions and discussions
GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

How to store GSH elution buffer for GST-tagged proteins purification ?

In order to keep my Tris 20 mM GSH pH8 elution buffer active for as long as possible I would like to know if I could prepare a large quantity and freeze it? (how much time can I freeze/thaw it?)...

19 February 2020 8,363 3 View

How are calculations of GSH and MDA concentrations in liver and kidney tissue of laboratory rats ??

Hi,,I need a method or equation for calculating the concentrations of Clutathione (GSH) and Malundaldehyde (MDA) in liver and kidney tissue of laboratory rats ...

16 February 2020 9,345 3 View

How to store GSH elution buffer for GST-tagged proteins purification ?

In order to keep my Tris 20 mM GSH pH8 elution buffer active for as long as possible I would like to know if I could prepare a large quantity and freeze it? (how much time can I freeze/thaw it?)...

01 February 2020 9,970 3 View

How to prepare a 20 mM reduced glutathione solution with pH = 7 ?

Hello, I would like to prepare a 20 mM reduced glutathione solution with pH=7. It's common to use PBS 1X to have this pH value. And in every publication, they "prepared GSH in PBS with pH=7,4"....

01 February 2020 3,013 3 View

How to express my results from GSH assay?

Hello, I am practicing GSH assay protocol (Rahman, 2006). I got the OD from the measurements, but I don't know how to express the results /mg of protein as the protocol mentions On the last table....

09 January 2020 9,965 3 View

How to express my results from GSH assay?

Hello, I am practicing GSH assay protocol (Rahman, 2006). I got the OD from the measurements, but I don't know how to express the results /mg of protein as the protocol mentions On the last table....

31 December 2019 7,265 3 View

Solvent to dissolve std reduced GSH ?

The literature and data sheets mention the water as the best solvent to dissolve reduced GSH, they also mention the oxidization of GSH in an aqueous medium. But few protocols suggest using Buffer...

11 December 2019 7,024 2 View

Does anyone measure GST in milk?

Im trying to assay GST activity in milk, but I cant find the optimus final concentrations of CDNB and GSH for this matrix. Does anyone knows wich contentrations are the better?

01 February 2019 3,916 4 View

Why would TCEP in water produce fluorescent products with ABD-F?

I am derivatizing GSH with ABD-F to make the glutathione fluorescent. I am using TCEP to reduce GSSG. When I run a blank control with just water (ultrapure) instead of GSSG the TCEP appears to...

31 December 2018 2,375 1 View

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