I have tried to fluorescently label graphene oxide sheets once with Fluorescein sodium salt and another time with Fluorescein isothiocyanate (FITC) by mixing 500 uM of Fluorescein (any of them) with 0.5 mg/ml GO overnight then washing with DI water 3X and then re-suspending the GO in DI water. After washing I take a drop of the suspension and place it on a glass slide, leave it to dry then look under the optical microscope using blue light (of wavelength 450-495nm) to excite it but I can't see it fluorescing while the Fluorescein on its own provide green fluorescence at this wavelength. I will be grateful to obtain any advice regarding how to fluorescently label GO sheets.
Thanks
Hy Yasmine graphene should not have many amine groups, so FITC would not label well.
Graphene sheets may do however have terminal -COOH, which can be labeled using EDC and an amine labeled fluorophore (see Pierce for further info on EDC).
So what do you recommend to use as a fluorescent dye to label the graphene oxide?
If you use EDC, any fluorophore with a free primary amino group will work.
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