How can i find very detailed explanations on various types of PCR?

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If someone says IR sensors are the same as MX sensors, do you accept it?

Somebody has told me Ir sensors that I have used in my circuit, are the same as Mx sensors however they are different and their circuits can not be arranged similar but he does not accept. Is...

06 June 2024 9,117 4 View

Do you have any research or invent about IR sensors?

I want to record a analog circuit between control and power circuits as innovation. My control circuit is IR sensors with bulb size 10mm (its max current to support power circuit is 100m[A] DC)and...

05 June 2024 4,085 4 View

Is it possible to use pair of IR sensors for lighting staircase?

Pair of IR sensors have sender and receiver sensors but some one declares it is possible to use this sensors for lighting stair case however I have used them for automatic liquid dropper and hand...

05 June 2024 8,302 1 View

How can preparation concentration ppm from Essential oils for plant?

We are studying about essential oil of some plant again of antimicrobial, we want use concentration by ppm, how can preparation this concentration and which solvent we are use. Thank you for any...

28 May 2024 2,085 3 View

What is the latest research in the world in the field of personality and artificial intelligence?

22 May 2024 1,070 3 View

How to get copyrights permission for free from Springer nature journals?

I changed the circumstances while ordering, but did not get free access. please help me if you have experience in this issue. thanks

19 May 2024 8,649 1 View

How does early exposure to a foreign language in infancy and childhood affect the unconscious language acquisition process?

Second language learners often experience immersion in real-life contexts with exposure to cultural nuances, diverse input sources, and strong intrinsic motivation. How about foreign language...

02 May 2024 8,307 2 View

Which unsolved problem or question you have in mind about chaos and chaotic systems?

If you are a researcher working with chaos and chaotic systems, which unsolved problem or question you have in mind Here I have collected some open problems and questions related to chaos and...

28 April 2024 8,493 2 View

How many times do we refill R290 cold storage systems per year, what is the leakage frequency of propane natural refrigerant?

Hello, I need the reference that shows the leakage rate or the refilling frequency of R290 refrigerant. Thank you

17 April 2024 5,298 0 View

Is this formulation correct for the linear Drucker-Prager criterion?

If the hydrostatic pressure is defined as p = -1/3 trace(σ), where σ is the Cauchy stress tensor, and under the assumption that Drucker-Prager failure surface circumscribes the Mohr-Coulomb...

13 April 2024 4,416 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

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05 August 2024 2,570 3 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Ciara Keating

Hi Mansour, I found this book super helpful to get the basics

https://www.routledge.com/Molecular-Microbial-Ecology/Osborn-Smith/p/book/9781859962831

Best wishes, Ciara

Mansour Jafari

Thank you Ciara, I really appreciate your help

Rosa Gallo

You could find ppt presentations on the universities' lectures. Perhaps, there is also something on biorad website.