I wanted to find the gene expression level of BEST1 gene (responsible for Best disease - occur in eye) in normal and diseased individuals or cells using GEO Omnibus - NCBI, but I don't know how to start exactly. I started with finding the specific gene sequence but in the middle of my experiment I stuck with it. Could somebody please advise?
If you go to GEO Profiles, you can type in BEST1 as your query. That will give you expression data for all experiments in GEO that measured BEST1 expression. Then it's just a matter of refining your query using the filters on the page, or by adding additional search parameters (e.g BEST1 AND "Homo sapiens").
If you know of a specific microarray experiment that was done in the literature, the GEO accession number is usually included in the publication and can be added to your query (e.g. BEST1 AND GSEXXXXX).
Hope that helps
If you go to GEO Profiles, you can type in BEST1 as your query. That will give you expression data for all experiments in GEO that measured BEST1 expression. Then it's just a matter of refining your query using the filters on the page, or by adding additional search parameters (e.g BEST1 AND "Homo sapiens").
If you know of a specific microarray experiment that was done in the literature, the GEO accession number is usually included in the publication and can be added to your query (e.g. BEST1 AND GSEXXXXX).
Hope that helps
If you need a ready made answer ,This will help you out
http://www.genecards.org/cgi-bin/carddisp.pl?gene=BEST1
best,
Ganesh
I don't know how committed you are to using GEO, however another good resource is http://ist.medisapiens.com/.
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