While it's well-known that many cancers rely heavily on glycolysis, are there cancer cell lines that depend more on mitochondrial respiration? What resources can I use to find this information?
Hello Yanxun Yu
Triple negative breast cancer (TNBC) cell lines such as MDA-MB-468 and MDA-MB-231 mainly exhibit enhanced OXPHOS. You may want to refer to the papers attached below for more information.
Article A gold-based inhibitor of oxidative phosphorylation is effec...
https://www.mdpi.com/1422-0067/21/10/3731
Article Oxidative Phosphorylation Is a Metabolic Vulnerability in Ch...
Article Energy Metabolism Drugs Block Triple Negative Breast Metasta...
https://www.nature.com/articles/s41416-020-01040-y
Best.
you can test cell growth and/mortality with culture media: One with a lot of glucose 4.5g/l(and glutamine), one with low- glucose 1g/l (and glutamine) and the last one without glucose (only glutamine). Glutamine will be transformed into alphaketoglutarate and fuel the krebs cycle, which forces the cell to live on mitochondria. For example, primary control fibroblasts can divide without glucose but with glutamine thanks to mitochondrial functions.
2 refs
- The glutamine-alpha-ketoglutarate (AKG) metabolism and its nutritional implications - PubMed (nih.gov) PMID 27161106
- Evolutionarily conserved susceptibility of the mitochondrial respiratory chain to SDHI pesticides and its consequence on the impact of SDHIs on human cultured cells - PubMed (nih.gov) PMID 31697708