How can I find KO IDs for ORF sequences in a large FASTA file?

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Abhijeet Singh

Using grep can be an option.

Aqib Sarfraz

Dear Sachin Chandrasekara You can use kofmKOALA (https://www.genome.jp/tools/kofamkoala/) to annotate amino acid sequences for KEGG ortholog (KO) ID. Since you have large datasets, so you can run it as a standalone application on your Linux terminal.

good luck

Ankur Das

Use EggNog mapper

https://github.com/eggnogdb/eggnog-mapper