18 Questions 13 Answers 0 Followers
Questions related from Sachin Chandrasekara
I have a metagenomic dataset consisting of paired-end and singleton FASTQ files, generated after host removal and quality filtering. Specifically: Forward reads: final_clean.1.fastq Reverse...
05 August 2025 7,958 3 View
Hi, I have a protein sequence file (about 14.9 GB) in FASTA format. Each sequence has an ORF ID in the header line. I want to find the KEGG Orthology (KO) IDs that match these ORFs. Can someone...
23 April 2025 414 3 View
I generated a network in R and exported it as a GraphML file (800 MB, over 1,000 nodes). Attempting to visualize it in Cytoscape causes crashes or freezes during import. Are there, Alternative...
20 March 2025 8,891 3 View
I have two question ? I’m experiencing complete PCR failure where no bands are visible (two scenarios) in any lanes after agarose gel electrophoresis. 1) Problem: No Bands in Any Lanes...
02 March 2025 1,568 9 View
I’m particularly interested in: Strategies to select variable regions (e.g., V3-V4 vs. V4-V5) for broad taxonomic coverage. Tools/software for in silico validation of primer specificity and...
10 February 2025 2,823 3 View
I recently performed PCR on 8 samples, including a positive control and a water control. Yesterday, I ran the PCR and subsequent gel electrophoresis, and I observed clear bands in all samples,...
29 January 2025 2,001 5 View
I am currently working on optimizing our inventory management system and need to calculate the monthly safety stock for various SKUs. I have already generated weekly safety stock values based on...
05 December 2024 8,447 2 View
Previously, my power app was working fine. However, after attempting to change the background images, it stopped functioning properly. When I submit a request (Form Screen) and then check its...
21 November 2024 5,140 1 View
I am working with a time series dataset using the `fable` package (R). I have fitted several models (e.g., ARIMA) and generated forecasts. The accuracy calculation is resulting in NaN values, and...
17 January 2024 7,732 2 View
I am working on a research project involving a system of differential equations with one ordinary differential equation (ODE) and two partial differential equations (PDEs). I would like to...
06 November 2023 5,965 4 View
I am conducting a research project involving the use of the MACD (Moving Average Convergence Divergence) signal indicator for analyzing multivariate time series data, possibly for trading...
12 October 2023 9,336 1 View
Need urgent help regarding this. I am currently working on a project involving multiple time series forecasting using R. For the forecasting process, I have monthly data available from 2019 up to...
16 May 2023 7,573 4 View
I have been assigned the task of performing business sales forecasting using time series analysis. However, before starting the forecasting process, I need to identify and treat the outliers in...
25 April 2023 3,910 4 View
I have a system of non-linear differential equations that explains the behaviour of some of the cancer cells. Looking for help identifying the equilibrium points and eigenvalues of this model in...
16 January 2023 805 2 View
I have been attempting to set up my Python workspace on VS Code, but the programme keeps telling me that I need to instal Python because it doesn't seem to recognise that I already have Python...
02 January 2023 3,989 6 View
I'm trying to write a java programme that will solve the system of ordinary differential equations using the Runge-Kutta fourth-order (RK4) technique. I need to solve a system of five equations....
02 December 2022 6,861 10 View
Using Latex, I want to insert the Google Scholar icon and Researchgate in my CV. I used the following command, but it doesn't appear researchgate icon. Link works....
10 November 2022 436 6 View
I am trying to do simple modelling similar to the basic tutorial given on the modeller's website following error occurs while I compile in the same directory. FATAL ERROR: Cannot open file...
28 October 2022 7,377 2 View
