17 Questions 13 Answers 0 Followers
Questions related from Sachin Chandrasekara
I have a metagenomic dataset consisting of paired-end and singleton FASTQ files, generated after host removal and quality filtering. Specifically: Forward reads: final_clean.1.fastq Reverse...
last month 7,922 3 View
I generated a network in R and exported it as a GraphML file (800 MB, over 1,000 nodes). Attempting to visualize it in Cytoscape causes crashes or freezes during import. Are there, Alternative...
20 March 2025 8,880 3 View
I have two question ? I’m experiencing complete PCR failure where no bands are visible (two scenarios) in any lanes after agarose gel electrophoresis. 1) Problem: No Bands in Any Lanes...
02 March 2025 1,562 9 View
I’m particularly interested in: Strategies to select variable regions (e.g., V3-V4 vs. V4-V5) for broad taxonomic coverage. Tools/software for in silico validation of primer specificity and...
10 February 2025 2,820 3 View
I recently performed PCR on 8 samples, including a positive control and a water control. Yesterday, I ran the PCR and subsequent gel electrophoresis, and I observed clear bands in all samples,...
29 January 2025 1,994 5 View
I am currently working on optimizing our inventory management system and need to calculate the monthly safety stock for various SKUs. I have already generated weekly safety stock values based on...
05 December 2024 8,431 2 View
Previously, my power app was working fine. However, after attempting to change the background images, it stopped functioning properly. When I submit a request (Form Screen) and then check its...
21 November 2024 5,133 1 View
I am working with a time series dataset using the `fable` package (R). I have fitted several models (e.g., ARIMA) and generated forecasts. The accuracy calculation is resulting in NaN values, and...
17 January 2024 7,710 2 View
I am working on a research project involving a system of differential equations with one ordinary differential equation (ODE) and two partial differential equations (PDEs). I would like to...
06 November 2023 5,959 4 View
I am conducting a research project involving the use of the MACD (Moving Average Convergence Divergence) signal indicator for analyzing multivariate time series data, possibly for trading...
12 October 2023 9,327 1 View
Need urgent help regarding this. I am currently working on a project involving multiple time series forecasting using R. For the forecasting process, I have monthly data available from 2019 up to...
16 May 2023 7,561 4 View
I have been assigned the task of performing business sales forecasting using time series analysis. However, before starting the forecasting process, I need to identify and treat the outliers in...
25 April 2023 3,899 4 View
I have a system of non-linear differential equations that explains the behaviour of some of the cancer cells. Looking for help identifying the equilibrium points and eigenvalues of this model in...
16 January 2023 792 2 View
I have been attempting to set up my Python workspace on VS Code, but the programme keeps telling me that I need to instal Python because it doesn't seem to recognise that I already have Python...
02 January 2023 3,973 6 View
I'm trying to write a java programme that will solve the system of ordinary differential equations using the Runge-Kutta fourth-order (RK4) technique. I need to solve a system of five equations....
02 December 2022 6,853 10 View
Using Latex, I want to insert the Google Scholar icon and Researchgate in my CV. I used the following command, but it doesn't appear researchgate icon. Link works....
10 November 2022 432 6 View
I am trying to do simple modelling similar to the basic tutorial given on the modeller's website following error occurs while I compile in the same directory. FATAL ERROR: Cannot open file...
28 October 2022 7,371 2 View
