Hello
I put a sample in the Time Resolved Flourescence. Then the instrument gave me a time-intensity curve. Now I want to find the decay time of the sample. If you know anything about these, please guide me.
Thanks
Dear Solmaz Kamrani ,
I will not repeat details; they are already written here as answers.
So please have for example a look at the answers of a similar question:
https://www.researchgate.net/post/Can_anyone_tell_me_the_graph_about_the_Time-Resolved_Photoluminescence_TRPL
From them you may pick up appropriate buzz words and google by yourself.
Good luck.
Usually one make log(intensity) plot vs time and makes linear fit to extract tau decay time constant. One may exclude initial area if it is distorted by the simulation or the tail of the emission if it is too weak and noisy. You may look at some examples here: https://www.horiba.com/fileadmin/uploads/Scientific/Photos/OSD/OBB/EasyLifeX/ANS-to-BSA_230px.jpg
If the decay is exponential (that is exp(-a.t)) then the decay time can be defined to be (1/a) that is time required to decay to 1/2.7 of the initial intensity.
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