I have found four SNPs on 3'UTR of a same gene. I would like to know where miRNA binding on 3'UTR what I have studied.
Please suggest any tool to find the miRNA binding site on 3'UTR.
Use mirdb: http://mirdb.org/
Hi!! I'm Yedukondalu K, and planing to do in silico analysis for protein. Previous, their is no protein modeling, 3D structure. So, how can I approach to do protein in silico analysis? . I'm...
11 December 2020 9,437 5 View
I'm using Swiss Model program package to model the wild-type and mutant variant. I have modelled the wild-type and mutant variant but I dont know how to write manuscript and how to validate after...
10 December 2020 9,769 3 View
is there any matlab code for tamper detection and recovery
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Hello everyone, My research work on to find out the novel and polymorphisms in selected gene.. I found few novel and SNP'S.. Now I am going to write a review article on SNP'S and their effect...
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One compound heterozygous SNP found in upstream ( g.IVS 06-68 C>T) and downstream ( g.IVS 06+13 A>G) intronic region of DNA. it may cause mutation/affect or not.
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In molecular crystals especially hydrogen bonded systems we often see red-/blue-shift in the IR/Raman spectra when they subjected to high pressure/temperature. When IR/Raman spectra show red- to...
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As per my understanding, noncovalent interactions play a crucial role in determining the stability of molecular crystals at ambient as well as at high pressure. When pressure increases, the...
26 February 2018 4,458 7 View
previous I optimised my designed primers without DMSO, but now my subject samples getting problem so I added DMSO, getting good intensity bands along with non specific bands. So now again...
14 June 2017 8,140 1 View
SNP’s were got in specific human gene which were sequenced but I want to know these SNP’s already submitted in Indian region, Asian and worldwide of specific targeted gene. So how could I check...
23 January 2017 4,165 0 View
First, I optimized the primers which were designed for specific exons, with different Temp (annealing Temp. of PCR) in gradient PCR with standard human blood DNA. After getting annealing Temp. did...
01 September 2016 1,242 5 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...
30 July 2024 9,824 2 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
I performed mmpbsa of protein-protein complex simulation on gromacs. My binding free energy is +166781. This complex is stable when experimentally produced in wet lab. In index.ndx I grouped...
19 July 2024 4,120 5 View
I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...
18 July 2024 2,881 4 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...
02 July 2024 5,502 4 View
I need CCN concentration over Arctic region, any observational dataset is welcomed. Thank you.
01 July 2024 6,082 0 View
Greetings mathematicians and physicists, As we know, the idea of assuming a higher space dimension than the natural 3 dimension is effective in solving many physical and mathematical...
29 June 2024 9,220 6 View