8 Questions 6 Answers 0 Followers
Questions related from Yedukondalu Kollati
Hi!! I'm Yedukondalu K, and planing to do in silico analysis for protein. Previous, their is no protein modeling, 3D structure. So, how can I approach to do protein in silico analysis? . I'm...
12 December 2020 9,379 5 View
I'm using Swiss Model program package to model the wild-type and mutant variant. I have modelled the wild-type and mutant variant but I dont know how to write manuscript and how to validate after...
11 December 2020 9,713 3 View
Hello everyone, My research work on to find out the novel and polymorphisms in selected gene.. I found few novel and SNP'S.. Now I am going to write a review article on SNP'S and their effect...
08 October 2019 9,177 3 View
One compound heterozygous SNP found in upstream ( g.IVS 06-68 C>T) and downstream ( g.IVS 06+13 A>G) intronic region of DNA. it may cause mutation/affect or not.
30 January 2019 3,066 3 View
previous I optimised my designed primers without DMSO, but now my subject samples getting problem so I added DMSO, getting good intensity bands along with non specific bands. So now again...
15 June 2017 8,075 1 View
SNP’s were got in specific human gene which were sequenced but I want to know these SNP’s already submitted in Indian region, Asian and worldwide of specific targeted gene. So how could I check...
24 January 2017 4,134 0 View
First, I optimized the primers which were designed for specific exons, with different Temp (annealing Temp. of PCR) in gradient PCR with standard human blood DNA. After getting annealing Temp. did...
02 September 2016 1,169 5 View
When I optimized my designed primer on human standard DNA it worked and gives the selected exon/PCR product. but its using for subject samples few samples are getting PCR product and few samples...
01 January 1970 380 3 View