Yedukondalu Kollati

6 Questions 6 Answers 0 Followers

Questions related from Yedukondalu Kollati

What are the software using to model for protein?

Hi!! I'm Yedukondalu K, and planing to do in silico analysis for protein. Previous, their is no protein modeling, 3D structure. So, how can I approach to do protein in silico analysis? . I'm...

12 December 2020 9,362 5 View

How to validate after modelled the wild-type and mutant variant by using the SWISS-PORT protein analysis?

I'm using Swiss Model program package to model the wild-type and mutant variant. I have modelled the wild-type and mutant variant but I dont know how to write manuscript and how to validate after...

11 December 2020 9,689 3 View

Which tools are available to get the data on nucleotide change and rs number?

Hello everyone, My research work on to find out the novel and polymorphisms in selected gene.. I found few novel and SNP'S.. Now I am going to write a review article on SNP'S and their effect...

08 October 2019 9,161 3 View

How can I avoid non specific bands while adding DMSO in pcr master mix? ?

previous I optimised my designed primers without DMSO, but now my subject samples getting problem so I added DMSO, getting good intensity bands along with non specific bands. So now again...

15 June 2017 8,052 1 View

How can I get good PCR product with subject DNA samples?

First, I optimized the primers which were designed for specific exons, with different Temp (annealing Temp. of PCR) in gradient PCR with standard human blood DNA. After getting annealing Temp. did...

02 September 2016 1,149 5 View

If DMSO added for amplification, will it give our specific product or other product?

When I optimized my designed primer on human standard DNA it worked and gives the selected exon/PCR product. but its using for subject samples few samples are getting PCR product and few samples...

01 January 1970 361 3 View