How can I extract proteins from the Trichoderma grown in PDB(Potato dextrose broth)? Can I check antimicrobial activity of these proteins/peptides?

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Negero Gemeda Negeri

Yes you can check antimicrobial activity of the peptides after characterizing the secondary metabolites you found. The following articles may help: plz look at the link

http://krishikosh.egranth.ac.in/bitstream/1/35337/1/pathology%201.pdf

Osmar Nascimento Silva

Hi Aqleem,

It is possible, take a look, in the article below. Are you thinking of doing a proteomic study? or evaluate whether this extract has activity?

https://www.omicsonline.org/an-efficient-method-for-extraction-of-secreted-proteins-of-a-filamentous-fungus-cryphonectria-parasitica-jpb.1000179.php?aid=1300

Khem Raj Meena

You can check antimicrobial activity of peptides and proteins. first you extract proteins by acid precipitation methods and check antimicrobial activity by Well diffusion method.