I have tried liquid- liquid extraction method for isolating the active compound for anti inflammation from active microbial broth by different organic solvents ethyl acetate, chloroform, hexane, dichloromethane, methanol and ethanol but none of them is capable for isolating it. These solvents were mixed with broth in 1:1 ratio and vigorously rotate for 2-4hours. What should I do to get the active compound? please help me.

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