what idea is used in fabrication of diagnostic kits (for example glucose kit) to avoid the effect of kit surface wetting on the electrochemical signal (like amperometry)? because as we know after dropping the blood on screen printed surface, we have fundamental changes in electrolyte medium and sometimes the signal is covered by turbulence caused by medium changes.
Dear Maedeh:
I am not sure if I understand clearly your question: You call weting effect the fact of put the blood drop on the electrode?
When you do this, many parallel processes occur. First, the electric contact between the electrodes is established and this results in a significant current. Additionally, blood is a very complex matrix and many parallel reactions can occur.
I suggest you make some experiments with water + electrolyte and water + electrolyte + glucose (at the same levels as present in blood. This will help you to understand better what is occurring on your electrode.
Best regards,
Lucio
Dear professor Angnes
thank you so much, I mean exactly the concept you have mentioned: "the electric contact between the electrodes is established and this results in a significant current". I am designing a coated screen printed electrode. in amperometric detection, low concentrations affected and covered by this turbulence even in water + electrolyte and water + electrolyte + glucose.
best regards
maedeh
dear anirban
i appreciate your answer. it will be useful of course. but I don't want to use enzymatic catalyst.
regards
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