How can I evaluate porcine pancreatic elastase activity in vitro?

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

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05 August 2024 3,084 6 View

ANY free software for reconstructing neurons in the microscopic image?

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04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

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04 August 2024 8,997 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How to isolate lymphocytes from mouse spleen?

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Why results of ROS flurescence are negative as there was no bacteria within?

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04 August 2024 5,974 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

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31 July 2024 193 4 View

What is the relationship between protein structure and N or C terminal tagging choosing?

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28 July 2024 366 3 View

Assistance with MTT Assay Data ?

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26 July 2024 7,511 3 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

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23 July 2024 8,492 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

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23 July 2024 4,603 4 View

How do we pick data for determination of Validation Acceptance Criteria?

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23 July 2024 7,292 3 View

Steingrimur Stefansson

Hi Carolina, I agree with Theo that there a lot of small substrates and proteinaceous elastase substrates available commercially.

http://www.sigmaaldrich.com/catalog/search?term=elastase+substrate&interface=All&N=0&mode=match%20partialmax&lang=en®ion=US&focus=product

Carolina Arruda de Faria

Thank you Theo Nikiforov and Steingrimur Stefansson for your valuable sugestions.