How is the charge distribution in a reverse biased pn junction when not in a closed circuit?

How charges are stored equally on both sides of a normal PN junction diode when it is reverse biased? For example if p side is connected to a -2 voltage supply and the N side being grounded ,won't...

27 April 2020 1,811 3 View

How to create a charged body(cloud) scenario in laboratory?

I want to measure the force/charge exerted on a body due to charged cloud from a distant place .Experts advise/answers are appreciated well in advance.

27 March 2018 5,462 2 View

How to calculate error and accuracy in adaboost algorithm(machine learning algorithm)?

need the explanation about the process being the classification. And the formula for error value and accuracy in calculation.

03 February 2018 8,496 3 View

Should we use RT primer when we are using Universal primers in RT-PCR?

11 November 2014 3,481 2 View

Theoretical study of DPPH radical scavenging activity - can anyone provide suggestions for further improvements?

21 July 2014 2,117 1 View

What is the meaning of antisolvent?

Solvents - miscible/immiscible

23 November 2011 2,691 12 View

Strength of electro-magnetic waves reduces by 1/square of distance from point of transmitting station.how you are planning to address this issue?

01 January 1970 4,632 4 View

Associating in Joint Research Studies?

Looking for likeminded researchers who are interested in joint research studies on Ecological Economics in India

01 January 1970 2,086 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View