Actually I want to isolate RNA from co cultured cell lines. Is it possible to enrich the quantity of RNA particularly from the bacteria?
Bacterial cells should be much smaller than their eukaryotic counterparts unless they form mycelia like streptomyces. In that case, simple filtration prior to RNA extraction might work.
You could try the MICROBEnrich kit.
The principle is simple, it removes polyadenylated RNA as well as ribosomal RNA (both eukaryotic and bacterial).
I have used other microbexpress kits for mRNA enrichment, not very impressed. But I never tried this one, that also removes polyadenylated mRNA to enrich bacterial mRNA. Probably worth a try.
Best of luck!
Vegard
The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA.
Thank you all for nice suggestions.......@ Vegard i will definitely try to use MICROEnrich kit but could you please let me know about other kits which you used and the best one as per as your uses.
Sure, Amit!
I have used ribozero. Works well for enriching mRNA from RNA. But they dont, as far as I know, have a kit for removing eukaryotic RNA (with polyA tales) from microbial....
Microbexpress does not work well for removing M tuberculosis rRNA, but probably works better for some other species, I haven't tried...
Cheers,
Vegard
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