I am looking for recommendations to effectively culture pesticide resistant bacteria. I tried with normal LB agar without pesticides and results are not satisfying as only one particular morphology was obtained.
Try it with NA media amended with the requisite doses of pesticide you are testing. Ensure only pure colony is taken. Add antibiotics as well to which your subject bacteria is resistant. Most contaminating bacterias will not grow due to antibiotics and pesticide you will add, just check the compatibility of pesticide and antibiotics first, proceed with culturing separately and then try merging both pesticide and antibiotics in a single plate. Take your observations early. If satisfied then store your plates at 4 degrees.
Good luck 👍
The question is if those pesticides have bactericidal properties. They probably don't. So a great majority of bacteria from those soils will be insusceptible rather than resistant. You're probably looking for pesticide-degrading bacteria...
Jakub Grzesiak Yes that's right. I am working on identification of such bacteria and one member of my team on insect gut bacteria of the same field, where the insect is resistant to the pesticide. I am looking for a good method for culturing those bacteria and if soil extract is required for culture as i need to buy it in a short delay.
The best media for cultivating bacteria from soil are 10% tryptic soy agar or R2A agar, the soil extract is optional. They support growth of a wide range of bacteria and fungi, so it would be difficult to find those decomposing a particular pesticide. The best thing to do is search the literature for a minimal salts medium and appropriate pesticide concentrations. You can inoculate agar directly with soil suspension or enrich the bacteria on a liquid medium with the pesticide and then spread plate it.
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