25 February 2021 4 4K Report

I want to run a variant caller on ASD sequences but I can't find any database that allows open access to it. Have we got something like SRA to download such data?

More Ritik Richard Asbjørn Kosælsen Sinha's questions See All
Electroactive surface area of bare and the synthesized material?

For electrochemical sensors, the electroactive surface area (ESA) show higher value for the working material as compared to bare (reported in several papers). My bare electrode is FTO and working...

17 January 2021 7,220 3 View

How can i select suitable gene sequence at Ring stage of Plasmodium Falciparum for primer design?

I am new to PCR. We are working on PCR based amplification of targeted DNA sequence of Plasmodium Falciparum. For that, We are targeting the Ring stage of the parasite. I want to know on which...

15 December 2020 6,954 3 View

How to calculate sample size?

The current study Digital Marketing in Indian and its Impact on Consumer behavior in post and Pre-covid-19 in Agra a district of the state utttar pradesh India has to be taken up . How should I go...

06 December 2020 5,614 4 View

No title

I have tried using PowerSoil Pro kit from Qiagen with slight modifications- incubating at 65 degree for 10 mins after adding solution CD1, increasing the number of washes with CD5 to 3 times and...

27 October 2020 7,782 3 View

Are oil glands present in sandalwood? Or it is a byproduct of sapwood to heartwood formation?

I came to know that there is no oilglands in sandalwood and it is a byproduct during transformation of sapwood to heartwood. Is it true?

04 October 2020 8,216 2 View

From where should I get the free climate data for downloading ?

Hello all, I need a small help for downloading free climate data sets for annual mean temperature, rainfall, flood and drought for period of 30 years of Bihar, India. Please suggest me some...

04 October 2020 1,939 19 View

Need CIF files (XRD) for iron ore sinter phases?

Hi, Can someone share the CIF file (XRD analysis) for SFCA, SFCA I, SFCAII, CF, Ca-Magnetite. Can't find these in COD Thanks, Moni

25 August 2020 7,377 2 View

How to identify the planes and directions in the pole figures obtained from TSL OIM Analysis 7?

I have provided the images reported in journal papers. Which technique in TSL OIM Analysis 7 is used to identify the planes and directions in the EBSD map? How is it colour coded with the pole...

03 August 2020 8,078 1 View

I would like to make my Research papers Private to uploaded in Research Gate. How can I do thins?

Dear Sir/Madam, I want to make Private the loaded research papers in the Research Gate . How can I do this? with regards Sincerely H N Sinha

23 July 2020 4,520 4 View

Can pearlite form (traces/locally) in an ultra-low carbon steel with a carbon percentage of <0.005%?

If the ultra-low carbon steel is held at high isothermal temperatures will it be possible to form pearlite? How can the formation of pearlite be confirmed other than morphological confirmation?

14 July 2020 9,251 4 View

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I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

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22 February 2021 2,968 1 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

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