I have salmon testis DNA in which I will disperse R6B dye molecule for fluorescence. My question is wherter I can dissolve the DNA in water or ehtanol and spin coat on silver sulbstrate.
Hello Dr. Cristiano Matricardi,
Salmon DNA is soluble in water but not in ethanol. If you are particular in using ethanol to spin coat, you may treat the DNA with a surfactant such as CTMA or BTMA ( DOI: 10.1063/1.2135205) before dispersing the dye molecule.
Pradeep
Dear Cristiano Matricardi,
I agree with Pradeep Chandran, The fabrication procedure is as follows:
DNA is water soluble but insoluble in organic solvents. To disperse in organic solvents, the DNA was modified by an ion-exchange reaction process through addition of cetyltrimethylammonium (CTMA) cationic surfactant. The resulting CTMA-modified DNA (CTMA-DNA) is soluble in organic solvent. The stepwise procedure for CTMA-DNA preparation is given here. 3 g of DNA fiber was dissolved in 1 l of deionized (DI) water with magnetic stirring at room temperature for 10 h. In a separate beaker containing 1 l of DI water, 6 ml of CTMA chloride solution was stirred at room temperature for 2 h. The CTMA surfactant was then added to the DNA solution slowly through a syringe followed by 3 h of stirring. The CTMA surfactant-modified DNA precipitate along with residual CTMA and NaCl was collected. Residual CTMA and NaCl were removed by filtration process. The filtered CTMA-DNA was dried overnight at around 50 °C to obtain CTMA-DNA powder. The CTMADNA powder thus prepared is ready for solvation in 1-butanol.
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