How can I dissolve casein in order to make a SCA medium?

More Lynne Itelson's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

Is it necessary to autoclave every vessel used in DNA isolation?

DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?

10 July 2024 7,526 3 View

Plant extract sterilization???

Dear researchers, I've been working on aqueous plant extract sample, then I autoclaved them in 10 min to sterilize and added some preservatives. However, some of them showed bacterial and fungal...

11 June 2024 1,040 2 View

Does autoclaving reduce the concentration of heavy metals in sewage wastewater?

I'm currently studying the treatment of sewage wastewater and am interested in understanding the impact of autoclaving on the concentration of heavy metals. Specifically, I would like to know if...

10 June 2024 2,579 4 View

I noticed some white growth in the phosphate buffer that was prepared just a week ago. What could be the cause of this, and how can it be resolved?

The buffer wasn't filtered.. Could this be the cause? Can we autoclave phosphate buffer to resolve this issue?

10 June 2024 3,970 2 View

Do I need to autoclave 2X CTAB and Wash buffer?

I am using CTAB method (Naser M. Salameh et al., 2014) for fungal DNA isolation and purification. During reagents preparation I am not sure weather I should autoclave 2X CTAB and Wash buffer. So...

09 June 2024 8,470 2 View

Could you please help me identify what might be wrong with my plaque assay?

I used Vero E6 cells and Mayaro virus. The virus was diluted in DMEM without FBS. The plaque media was 2X MEM, prepared with sodium bicarbonate and L-glutamine, adjusted to a pH of 4 with sodium...

03 June 2024 519 0 View

How can I clean autoclave (PTFE) from sulfur contamination ? in all aspect of color, smell and existence of Nano particle of sulfur??

the autoclave has been smelly and seem likely yellow after my synthesis and this changes may effected on other synthesis, so I want to clean PTFE from all sulfur contamination that remain on...

31 May 2024 2,985 0 View

Biobase BKQ-B120L autoclave issues?

I am trying to get a new Biobase BKQ-B120L autoclave to work however it does not go through its cycles. It will start up and go to about 110C and then remains there untill the ERR alarm sounds....

21 May 2024 4,886 1 View

Chinaza Godswill Awuchi

Check https://microbiologyinfo.com/starch-casein-agar-sca-composition-principle-uses-preparation-and-result-interpretation/

Also check https://www.freepatentsonline.com/2200353.html

Kiprotich Kiptum

Check on this paper.

https://link.springer.com/protocol/10.1007/978-1-0716-1724-3_16