RNA was extracted using a standard acid guanidinium thiocyanate-phenol-chloroform approach.
I need urgent help with this please, thanks
I use a 'direct-zol' kit from Zymo research which yields excellent quality RNA, even from small tissue samples. I think there is guidance in the manual to optimise for bacterial samples too.
http://www.zymoresearch.com/rna/direct-zol
Well thank you for that, but I'd just need the protocol procedures for it, and I can't find any manuals.
2. mRNA extraction.
RNA was extracted using a standard acid guanidinium thiocyanate-phenol-chloroform approach.
Devise a protocol to allow the extraction of mRNA from a 10 ml culture of Escherichia coli.
Guanidium thiocyanate phenol chloroform methods are more or less summarised as "TRIzol", so generic usable (but non-optimised) protocol here.
http://tools.lifetechnologies.com/content/sfs/manuals/trizol_reagent.pdf
Agree. Here is a summarised protocol for an 'Ultraspec' kit including optimisation for bacteria. The ultraspec reagent is a guanidinium thiocyanate-based denaturant.
http://www.ecogen.com/upfiles/F11812.pdf
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