Jason Hughes

6 Questions 11 Answers 0 Followers

Questions related from Jason Hughes

How do I count number of irons in haemoglobin?

I want to do an experiment counting number of iron in haemoglobin and compare it to the original (which is 4). I need a protocol to do that please. Any uploads or links? Would be urgent, thank you.

13 April 2015 3,590 2 View

Where can I find a protocol to determine how much iron is present in each molecule of haemoglobin?

Ferrozine (disodium 3-(2-pyridyl)-5,6-bis(4-phenyl sulphonate)-1,2,4-triazine) is a water-soluble Fe(II) chelator. When chelated to iron, the complex absorbs light at 562 nm (molar absorption...

29 March 2015 2,814 7 View

Which statistical method should I use?

I want to do an experiment on comparing bathroom door handles of women and men to see if men are dirtier than women and if they tend to wash their hands less. I would just swab different door...

25 February 2015 1,472 8 View

Why is it that alkaline phosphatase, unlike most other enzymes, is not inactivated by the treatment with SDS?

So I did an SDS gel electrophoresis and wonder why alkaline phosphatase doesn't get inactivated by SDS compared to other enzymes, thanks.

22 February 2015 6,897 6 View

Is there a protocol to deduce the specific activity of LacZ in a 10 ml culture sample of Escherichia coli?

LacZ activity was assayed using a colorimetric assay based on the reaction of LacZ with ONPG (ortho-nitrophenyl--galactoside), with product concentration measured at 420 nm. To allow calculation...

09 February 2015 8,826 6 View

How can I devise a protocol to allow the extraction of mRNA from a 10 ml culture of Escherichia coli?

RNA was extracted using a standard acid guanidinium thiocyanate-phenol-chloroform approach. I need urgent help with this please, thanks

07 February 2015 4,125 6 View