i want to test for the efficacy of a plant extract in neutralizing the venom of a particular snake
J Nat Sci Biol Med. 2011 Jul-Dec; 2(2): 198–204.doi: 10.4103/0976-9668.92326PMCID: PMC3276014
Anti-cobra venom activity of plant Andrographis paniculata and its comparison with polyvalent anti-snake venom
Abstract
Background:To investigate the anti-cobra venom effect of alcoholic extract of Andrographis paniculata.
Materials and Methods:After calculating the LD99 of snake venom, the venom-neutralizing ability of plant extract at the dose 1 g/kg and 2 g/kg was determined using in vitro and in vivo methods. The alleviation in the mean survival time of the animals were used to infer the antivenom property of the drug after challenging with LD99 of snake venom.
Results:The ethanolic extract of plant A. paniculata significantly increases mean survival time and the protection fold, but could not protect animals from death when used alone. The higher dose, i.e., 2 g/kg was found better than that of the lower. ASV was found more effective than the plant extract. When ASV was given along with plant extract, it potentiates its effect.
Conclusion:The observation demonstrates the anti-cobra venom activity of ethanolic extract of A. paniculata which is comparable with ASV.
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Dear Musa,
During ethnobotanical field tours to various tribal dominated areas in West Bengal, India I found that various plants are used by them as an antidote to snake bite. I wish best of luck in your research work. If you can clinically identify even a single plant effective in snake bit, it would be of great help to the society.
Some study reports available for in vitro efficacy of plant extracts to nutralise some snake venom. But those all failed in animal experimentation. So, my advise is to test directly your plant extract/s directly on animal model (mice preferably) under the guidance of some experienced experts. Ethical permission is also required for such experiments.
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