There are several methods to determine the concentration of hydrogen peroxide in solution. I heard that DPD method and using coumarin are often used method.
Can you explain these method more specific? And are there other ways to determine hydrogen peroxide?
Dear Kyoung, please, use Amplex Red. Up to date it is the most reliable way.
Good luck!
Hi Kyoung,
I agree with Stanislovas. Amplex Red assay (https://www.thermofisher.com/order/catalog/product/A22188?SID=srch-srp-A22188) is worth to try. Both spectrophotometer and fluorometer can be used to run the assay in microplate format and it is sensitive enough to detect low concentrations of hydrogen peroxide.
i know I2 can be used in titration of peroxyacid so it might work the same for hydrogen peroxide.
Use the old method of titration with an aqueous solution of potassium permanganate. This was how manufacturers of H2O2 determined the concentration of hydrogen peroxide. I'm sure you can find the procedure in the literature.
Dear Kyoung,
I can suggest two methods which we have used for determination of hydrogen peroxide. A quick and approximate measurement can be made by peroxide test strips (Part#: 33815-P100) from Indigo Instruments. For a precise quantitative analysis, the iodate/UV-VIS spectroscopy method is suggested. The reference is:
N. V. Klassen, D. Marchington, H. C. E. McGowan, Anal. Chem. 1994, 66,
2921 –2925
This reference also describes the KMnO4 titration method.
Best,
Arman
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