I performed ethanol extraction using 50 g of Nigella sativa with 250 ml ethanol and 100 g of turmeric with 500 ml ethanol. After filtration, we proceeded directly to nanoformulation without evaporating the ethanol solvent. The nanoformulation was prepared as follows:

• Stock solution: 3 ml acetic acid + 300 ml distilled water
• TPP solution: 0.05 g TPP + 50 ml distilled water (for each formulation)

Since we did not evaporate the ethanol solvent before nanoformulation, how can I determine the concentration of crude extract in each nanoformulation to proceed with the MTT assay?"