How can I determine the concentration of cocktail I will use is enough to inhibit proteolytic activity?

20 June 2019 1 8K Report

I wanna perform homogenization for western blotting in rat brain tissue. But I wonder about which concentration protease inhibitory cocktail (Takara, cat no: 635673) I will use? or how can I determine the concentration of cocktail I will use is enough to inhibit proteolytic activity?

Thank you so much.

Steingrimur Stefansson

Hi Enis Hidisoglu . The 100X Takara coctail looks good, dilute to 1X. The most labile component is PMSF, but it should be stable since it is in DMSO.

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