The following literature can be reviewed

1)Methods in Biotechnology, Vol. 11: Aqueous Two-Phase Systems: Methods and Protocols; Edited by: R. Hatti-Kaul © Humana Press Inc., Totowa, NJ

2)PHASE COMPOSITIONS OF AQUEOUS TWO-PHASE SYSTEMS

FORMED BY POLY (ETHYLENE GLYCOL) AND MALTODEXTRIN

AT DIFFERENT TEMPERATURES by D. Ramyadevi, A. Subathira and S. Saravanan*

International Journal of Chemical Sciences and Applications

ISSN 0976-2590, Online ISSN 2278 – 6015

Vol 3, Issue 2, 2012, pp 289-292

3)Purification of plasmid DNA with aqueous two phase systems

of PEG 600 and sodium citrate/ammonium sulfate

Gabriela A. Gomes, Ana M. Azevedo, M. Raquel Aires-Barros, D. Miguel F. Prazeres

Separation and Purification Technology 65 (2009) 22–30

4) Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems ;Journal of Chemistry; Volume 2013 (2013), Article ID 938154, 6 pages

Zhiwen Bai,1,2 Yanhong Chao,2 Meiling Zhang,2 Changri Han,1 Wenshuai Zhu,2 Yonghui Chang,1 Huaming Li,2 and Yang Sun2

Thanks for your guidance

In cloud point method when we titrate the stock solution1 with stock solution2 how much it takes to see the cloudy point?

The binodal curve can be determine by (i) drop-wise addition of an aqueous solution of the salt to polymer aqueous solution (or pure polymer) until the detection of a cloudy  and biphasic solution, or vice versa; (ii) drop-wise addition of ultra-pure water until the formation of a clear and limpid solution corresponding to the monophasic regime.The

composition of the two-phase system can be determined by the ratio of the weight of a component added to the total weight of all components added. The above procedure can be repeated till we observed least amount of turbidity to obtain sufficient data for binodal curve

you can refer latest literature related to ABS of ionic liquid + inorganic salt