Please go thorugh the artcile
Phosphomonoesterase, phosphodiesterase, phosphotriesterase, and inorganic pyrophosphatase activities in forest soils in an alpine area: effect of pH on enzyme activity and extractability by R. Margesin and F. Schinner
BIOLOGY AND FERTILITY OF SOILS
Volume 18, Number 4 (1994), 320-326, DOI: 10.1007/BF00570635
Dear Colleague, p-nitrophenyl phosphate and bis(p-nitrophenyl) phosphate are the reagents of choice for the determinations you have requested. After enzyme action, you will check p-nitrophenol production by a mild alkaline treatment, changing p-nitrophenol into its intensely yellow anionic form (lambda max around 400 nm). Obviously a subtractive procedure should be adopted as the product arising from phosphodiesterase reaction is a substrate for the phosphatase, if present.
Thank you for your responses. I will review all suggestions and get beck to you all.
Ok, try.
TABATABAI, M. A.; BREMNER, J. M. Arylsulfatase activity of soils. Soil Science Society of America Proceedings, Madison, v. 34, p. 225-229, 1970
Thank you very much Elaine. I will try and search for it. Please i will appreciate it if you can send a copy of the article to me if you have the soft copy via my e-mial: [email protected]. Thank you very much.
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