We want to describe whether cotyledon number variation within species is associated to polymorphic microsatellite and whether polymorphic microsatellite can be different between di- and three cotyledon individuals and whether nucleotide number and repeat number are different among populations within species.
Dear, Amartuvshfin
Yes you can do a comparative study (it is certain you will find a polymorphism between the two taxa) between populations di and tri cotyledon by a fairly large number of microsatellites (the interval between markers must be
1. For your title question--"How can I detect Polycotyledon (POC) gene on cotyledons?"
For this, you need to develop a mapping population to looking for 'markers' which closed link to this POC locus. Once you have those very closed linked markers, you can use these marker for 'chromosome working' to the locus. Similar study has been done on tomatoes. See this paper: "Genetic characterization of the polycotyledon locus in tomato" [ http://www.ncbi.nlm.nih.gov/pubmed/16807733 ].
2. You may find microsatellite groups are different between bi- and tri-cotyledon plants, but this cannot be warrantied as the reason which cause the different numbers of cotyledons. It can be other genes involved. You need more studies. Attached is a paper about different types of repetitive sequences in different plant genome. It may be useful for you.
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