Using multiple linear regression to solve a multiple-way ANOVA problem?

Specifically, I want to know if I can use multiple linear regression to predict a response value 'y' from it's associated "treatment" or "group" averages in several variables. Let's say:...

04 November 2020 632 19 View

Somebody know a fast DNA extraction method for plant for to do in the field?

I am looking found reference about people that has been working the proposed method in this publication: "Nucleic acid purification from plants, animals and microbes in under 30 seconds" from Zou...

10 March 2020 860 2 View

Do viruses mutate more when transmitted to another host than when staying within the first host?

I would like to know if there is a published work studying the within-host mutation rate of a virus versus the between-hosts mutation rate, when a direct transmission event occurred between those...

13 November 2019 5,488 5 View

No title

I have generated a stably transfected SK-N-SH cell line that expresses Amyolid beta 42 fused to the pre-proncephalin signal peptide under de CMV promoter. I checked expression though qPCR and...

23 July 2019 2,685 2 View

Is there a way in matrix algebra to define the operations you want to perform on matrices???

Let's say I want to perform the conventional matrix multiplication, so I define it as "multiply then sum the products" (i.e. "x,+") For example, for matrices A and B: if A = |a b| .........|c...

21 February 2018 6,474 3 View

How to perform an ELISA sandwich with Chylomicrons?

18 September 2017 8,336 8 View

How to perform an ELISA sandwich with Chylomicrons?

08 September 2017 4,123 8 View

Bio-Rad Criterion tris-tricine gel electhophoresis. Which buffer to use?

03 April 2017 9,839 3 View

How to calculate mass of dry Tris base + Tris HCl to dissolve to give a buffer with desired concentration?

I am trying to make a 1M Tris Cl pH8 buffer with dry Tris base and Tris HCl. I know that it is possible to make Tris buffers without the use of HCl by combining powdered Tris acid with Tris base....

02 April 2017 695 4 View

Which are the mechanism that could increase the number of d(GT) repetition in DNA?

How GT dinucleotides repetitions are increased in an microsatellite? Is the replication machinery involved in this phenomenon?

16 January 2017 4,612 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View