Hi, all:
I want to cut the target sequence from one shRNA vector (PLKO) to ligand to another vector to generate a inducable system, I want to use the same restriction enzyme, but the question is one of the enzyme site was destroyed when generate the original plasmid, so what can I shoud do, can I still digest and isolate it?
Raheela Aslam
if one restriction site is deleted then how could u digest it after isolation? isolation wil gives a linear plasmid in this case, if em not wrong. and u could't ligate it properly with some other vector
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