Jun Zhang

3 Questions 4 Answers 0 Followers

Questions related from Jun Zhang

How can I cut the target sequence from one shRNA vector and ligand to another vector using the same restriction enzymes?

Hi, all: I want to cut the target sequence from one shRNA vector (PLKO) to ligand to another vector to generate a inducable system, I want to use the same restriction enzyme, but the question is...

04 April 2016 3,476 1 View

Does the exogenous mutant plasmids affect the endengous gene's function?

I want to study the function of my interested kinase, and now I want to use dot mutant skill to block the kinase site. Since I do not have this gene knockout cell model, so if I transfect the dot...

03 March 2016 5,098 3 View

What are the strategies to avoid the Heavy/Light chains in co-IP assay?

Does anyone have experience to avoid the IgG heavy/Light chains in immunoprepication assay to detect the endogenous targets? Because my target protein MW is close to the heavy chain (about 60 kd)....

01 January 2016 2,264 3 View