Can anyone suggest for me an appropriate way to culture autohydrogenotrophic bacteria? Can I collect sludge from SBR reactor to culture it?
Different autohydrogenotrophic bacterial strains can be cultured using Himedia
nutrient broth [13.0 g/l distilled water: composed of (g/l): peptic digestion of animal tissue 5.0, NaCl 5.0, beef extract 1.5 and yeast extract 1.5] and incubated at 37C at 200 rpm for 16–20 h.
SLUDGE SAMPLE MAY BE USED FOR ISOLATION OF AUTOHYDROGENOTROPHIC BACTERIA.
For batch-culture, 250 ml of different concentrations (0.5, 1.0 and 2.0%) of glucose in (i) GM-2 medium composition g/l (yeast extract, 1.0; K2HPO4, 1.0 and
MgSO47H2O, 0.5) and (ii) minimal medium (M-9) of composition 1x/l (Na2HPO4, 6.0 g; KH2PO4, 3.0 g; NaCl, 0.5 g; NH4Cl, 1.0 g; MgSO4, 1.0 mM and CaCl2, 0.1 mM) were added in 300 ml BOD bottles. pH of the glucose containing medium was adjusted to 7.0. The bottles with then flushed with argon and incubated at 37C. The
evolved gases were collected by water displacement method and residual glucose was measured by DNSA method. The process of gas collection and analysis was
continued until H2 evolution ceased.
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