I would like to make the graph of absorption spectrum, which can only be viewed or printed out from the UV 1800. Any ways of converting theraw data files with specific extension of PKCS#7, may kindly be suggested.
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Hi, I have grown BL21 cells with the pET28a that carries my insert sequence. It is growing on the selection plate but I can't observe any insert sequence bands when I do colony PCR. Is there any...
15 July 2024 5,135 5 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
I have harvested human dermal fibroblast cells and subcultured into T25 flasks using fibroblast expansion medium that contant 10% LSGS and 1% anti-anti. When observe the cells a day after...
16 May 2024 8,239 0 View
I am working on cold spray technology and currently doing validation for discrete phase modelling. The total mass flow rate and initial velocity of the particles is not mentioned in the paper...
08 April 2024 9,612 4 View
I am currently validating a paper on Cold Spray technology and simulating the particle flow using 'Discrete Particle Modelling'. I got the particle tracks and the particle history data, but...
03 April 2024 9,465 2 View
I will be using the Himedia PCR cleanup protocol and kit, so if anyone has any hacks to reduce the smear, do let me know. I will be sending samples urgently for sequencing. The sequencing is...
03 January 2024 6,635 3 View
We grow the microbe in complex sugar media, spin it, take the supernatant, and add buffer and water. In the case of a test, we add the substrate and incubate it before adding DNSA, bringing it to...
05 October 2023 6,303 0 View
Hi, I am working on protein-protein interaction studies, specifically on antibody-antigen interaction. I would like to observe the changes in interaction if there's mutation occurs in the protein....
04 October 2023 8,796 3 View
Hello, I am working on microRNA expression studies on Regeneration. I isolated RNA from my sample and converted it into cDNA using Poly A polymerase and Reverse transcriptase enzyme. Previously I...
18 August 2023 3,898 0 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....
31 July 2024 3,843 7 View
How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...
28 July 2024 9,165 2 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
i need to find ECC for 4-MIMO antenna using radiation pattern on CST
16 July 2024 7,291 1 View
Hi, I need to measure the real-time (or temporal intervals) thickness variation of a transparent material from dry to wet/humid conditions, however, I cannot use the following techniques: -...
08 July 2024 4,799 4 View
I'm attempting to simulate a crossed dipole antenna. For excitation, I've placed a lumped port along each dipole. However, the resulting radiation pattern is similar to that of a single dipole....
08 July 2024 1,876 3 View